Spectrophotometric method validation report for determination of cyanide in water Parameters: Test standard for determination of cyanide in water: HJ484-2009
Equipment: Intelligent integrated distillation instrument Equipment model: ST106-3RW
Instrument conditions: Intelligent integrated distiller: Heating power 500W (full boiling mode); set volume 85mL; time setting 60min.
Experimental time: November 12, 2016 1. Method validation steps
1.1 Reagent preparation: Prepare according to HJ484-2009.
1.2 Drawing of standard curve: Draw according to the operating steps specified in HJ484-2009.
1.3 Precision experiment: Use standard material for analysis of cyanide content in water (the standard material production unit is China Institute of Metrology, and the standard material batch number is 16098) to prepare three gradients of water samples with high, medium and low concentrations, and measure each sample six times in parallel. Calculate the relative standard deviation of the six samples at each concentration.
1.4 Accuracy test: The total cyanide standard sample in water (the standard sample production unit is the Standard Sample Research Institute of the Ministry of Environmental Protection, and the standard material batch number is 202260) is used for the test. Carefully open the ampoule before use, accurately measure 10mL of the concentrated sample from the ampoule into a 1000mL volumetric flask with a 10mL dry and clean pipette, dilute to the scale with 0.1% sodium hydroxide solution, mix well and use immediately. Take 100mL of the diluted standard sample solution to prepare 6 parallel samples, and conduct the experiment according to the experimental steps of HJ484-2009. Calculate the concentration of the standard sample and determine whether it is within the error allowable range. 484-2009) detection requirements.