The step of measuring a low concentration of the anionic surfactant of the present method is applicable to surface and ground water, a sewage treatment plant or clean water. 10ml chloroform is used in the system. Instrument Model: Jinan Shing automated liquid-liquid extraction apparatus, Model: STC-302B.

experiment procedure:
1. The 100ml sample was transferred to a separatory funnel 250ml, if a higher concentration of the sample, the test amount taken was diluted to 100ml.
2. 25ml of methylene blue, mixing, extraction instrument to set the intensity 50Hz, 90s time, allowed to stand 20min.
3. Add 10ml of chloroform, and extracted intensity of 25Hz, the extraction time was 90s, spaced 30s, 2 times, standing layer.
4. funnel with filter paper for drying divided liquid pipe wall, a small plug of cotton League Cadres in diameter, the chloroform layer cuvette, the absorbance at 652 nm was measured by a dry sample to the cotton wool 10mm chloroform as the reference.
The blank test, only water instead 100ml sample.

That test procedure

The step of measuring a high concentration of the anionic surfactant present method applies to both low concentrations of surface and ground water, but also for sewage, waste water and industrial waste water medical assays. 25ml chloroform is used in the system.
Instrument: automatic Jinan Shing liquid-liquid extraction apparatus, Model: STC-302B.
experiment procedure:
1. The 100ml sample was transferred to a separatory funnel 250ml, if a higher concentration of the sample, the test amount taken was diluted to 100ml.
2. 25ml of methylene blue, mixing, extraction instrument to set the intensity 50Hz, 90s time, allowed to stand 20min.
3. Add 25ml of chloroform, and extracted intensity of 25Hz, the extraction time was 90s, spaced 30s, 3 times, standing layer.
4. funnel with filter paper for drying divided liquid pipe wall, a small plug of cotton League Cadres in diameter, the chloroform layer cuvette, the absorbance at 652 nm was measured by a dry sample to the cotton wool 10mm chloroform as the reference.
The blank test, only water instead 100ml sample.

That test procedure

Instrument: automatic liquid-liquid extraction apparatus, Model: STC-302B